Article
DnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 2 Apr 2002
Echave Pedro, Esparza-Cerón M Angel, Cabiscol Elisa, Tamarit Jordi, Ros Joaquim, Membrillo-Hernández Jorge, Lin E C C
Abstract excerpt
The adhE gene of Escherichia coli encodes a multifunctional ethanol oxidoreductase (AdhE) that catalyzes successive reductions of acetyl-CoA to acetaldehyde and then to ethanol reversibly at the expense of NADH. Mutant JE52, serially selected for acquired and improved ability to grow aerobically on ethanol, synthesized an AdhE(A267T/E568K) with two amino acid substitutions that sequentially conferred improved...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.