Article
Ca2+- and S1-induced conformational changes of reconstituted skeletal muscle thin filaments observed by fluorescence energy transfer spectroscopy: structural evidence for three States of thin filament.
Journal of biochemistry - 1 Mar 2002
Hai Hong, Sano Ken-Ichi, Maeda Kayo, Maéda Yuichiro, Miki Masao
Abstract excerpt
Rabbit skeletal muscle alpha-tropomyosin (Tm) and the deletion mutant (D234Tm) in which internal actin-binding pseudo-repeats 2, 3, and 4 are missing [Landis et al. (1997) J. Biol. Chem. 272, 14051-14056] were used to investigate the interaction between actin and tropomyosin or actin and troponin (Tn) by means of fluorescence resonance energy transfer (FRET). FRET between Cys-190 of D234Tm and Gln-41 or Cys-374...
Topics
- Actin Cytoskeleton
- Actins
- Animals
- Calcium
- Energy Transfer
- Gene Deletion
- Molecular Probes
- Muscle, Skeletal
- Mutation
- Myosin Subfragments
- Protein Conformation
- Rabbits
- Spectrometry, Fluorescence
- Tropomyosin