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Article

A three-dimensional FRET analysis to construct an atomic model of the actin-tropomyosin complex on a reconstituted thin filament.

Journal of molecular biology - 16 Dec 2011

Miki Masao, Makimura Satoshi, Saitoh Takahiro, Bunya Masashi, Sugahara Yasuyuki, Ueno Yutaka, Kimura-Sakiyama Chieko, Tobita Hidetaka

Abstract excerpt

Fluorescence resonance energy transfer (FRET) was used to construct an atomic model of the actin-tropomyosin (Tm) complex on a reconstituted thin filament. We generated five single-cysteine mutants in the 146-174 region of rabbit skeletal muscle α-Tm. An energy donor probe was attached to a single-cysteine Tm residue, while an energy acceptor probe was located in actin Gln41, actin Cys374, or the actin nucleotide...

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Source

Identifiers

PMID: 22051514
DOI: 10.1016/j.jmb.2011.10.033

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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