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Article

Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis.

European journal of biochemistry - 1 Feb 2002

Bokma Evert, Rozeboom Henriëtte J, Sibbald Mark, Dijkstra Bauke W, Beintema Jaap J

Abstract excerpt

Hevamine is a chitinase from the rubber tree Hevea brasiliensis. Its active site contains Asp125, Glu127, and Tyr183, which interact with the -1 sugar residue of the substrate. To investigate their role in catalysis, we have successfully expressed wild-type enzyme and mutants of these residues as inclusion bodies in Escherichia coli. After refolding and purification they were characterized by both structural and...

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PMID: 11846790
DOI: 10.1046/j.0014-2956.2001.02721.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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