Article
Structural and functional analysis of de-N-acetylase PgaB from periodontopathogen Aggregatibacter actinomycetemcomitans.
Molecular oral microbiology - 1 Aug 2017
Parthiban C, Varudharasu D, Shanmugam M, Gopal P, Ragunath C, Thomas L, Nitz M, Ramasubbu N
Abstract excerpt
The oral pathogen Aggregatibacter actinomycetemcomitans uses pga gene locus for the production of an exopolysaccharide made up of a linear homopolymer of β-1,6-N-acetyl-d-glucosamine (PGA). An enzyme encoded by the pgaB of the pga operon in A. actinomycetemcomitans is a de-N-acetylase, which is used to alter the PGA. The full length enzyme (AaPgaB) and the N-terminal catalytic domain (residues 25-290, AaPgaBN)...
Topics
- Acetylesterase
- Aggregatibacter actinomycetemcomitans
- Bacterial Proteins
- Biofilms
- Catalytic Domain
- Crystallization
- Crystallography, X-Ray
- Escherichia coli Proteins
- Kinetics
- Metals
- Models, Molecular
- Mutation
- Operon