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Article

Structural dynamics of the Ca2+-regulated cutinase towards structure-based improvement of PET degradation activity.

International journal of biological macromolecules - 1 Nov 2024

Numoto Nobutaka, Kondo Fumiya, Bekker Gert-Jan, Liao Zengwei, Yamashita Mitsuaki, Iida Akira, Ito Nobutoshi, Kamiya Narutoshi, Oda Masayuki

Abstract excerpt

We previously revealed the structural basis of Ca2+ dependent regulation of a polyethylene terephthalate (PET)-degrading enzyme, Cut190, and proposed a unique reaction cycle in which the enzyme repeatedly binds and releases Ca2+. Here, we report crystal structures of Cut190 mutants with high thermal stability complexed with PET-like ligands that contain aromatic rings. The structural information has allowed us to...

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PMID: 39419144
DOI: 10.1016/j.ijbiomac.2024.136597

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