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Article

Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center.

Biochimica et biophysica acta - 1 Apr 2014

Ohnuma Takayuki, Umemoto Naoyuki, Nagata Takuya, Shinya Shoko, Numata Tomoyuki, Taira Toki, Fukamizo Tamo

Abstract excerpt

DESCRIPTIONS: The structure of a GH19 chitinase from the moss Bryum coronatum (BcChi-A) in complex with the substrate was examined by X-ray crystallography and NMR spectroscopy in solution. The X-ray crystal structure of the inactive mutant of BcChi-A (BcChi-A-E61A) liganded with chitin tetramer (GlcNAc)4 revealed a clear electron density of the tetramer bound to subsites -2, -1, +1, and +2. Individual sugar...

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PMID: 24582745
DOI: 10.1016/j.bbapap.2014.02.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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