Article
In vitro differentiation of WB-F344 rat liver epithelial cells into the biliary lineage.
Differentiation; research in biological diversity - 1 Jan 2002
Couchie Dominique, Holic Nathalie, Chobert Marie-Noële, Corlu Anne, Laperche Yannick
Abstract excerpt
Differentiation of hepatic precursor cells in the biliary lineage has rarely been investigated, owing to the lack of convenient in vitro models. In this study, we used sodium butyrate and culture on Matrigel to promote differentiation of WB-F344 rat liver epithelial cells along the biliary phenotype. This differentiation was assessed by following the expression of phenotypic markers at the protein or mRNA level....
Topics
- Animals
- Bile Ducts
- Biomarkers
- Butyrates
- Cell Differentiation
- Cell Division
- Cell Line
- Cell Lineage
- Collagen
- Drug Combinations
- Enzyme Induction
- Epithelial Cells
- Keratins
- Laminin
- Liver
- Phenotype
- Proteoglycans
- RNA, Messenger