Article
Hepatitis C virus (HCV) E1 and E2 protein regions that specifically bind to HepG2 cells.
Journal of hepatology - 1 Feb 2002
Garcia Javier Eduardo, Puentes Alvaro, Súarez Jorge, López Ramses, Vera Ricardo, Rodríguez Luis Eduardo, Ocampo Marisol, Curtidor Hernando, Guzman Fanny, Urquiza Mauricio, Patarroyo Manuel Elkin
Abstract excerpt
BACKGROUND/AIMS: Identify hepatitis C virus (HCV) sequences in E1 and E2 protein binding to HepG2. METHODS: Synthetic 20-mer long, ten-residue overlapped peptides, from E1 and E2 proteins, were tested in HepG2 or Raji cell-binding assays. Affinity constants, binding site number per cell and Hill coefficients were determined by saturation assay for high activity binding peptides (HABPs). Receptors for HepG2 cell...
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