Article
Phage-display evolution of tyrosine kinases with altered nucleotide specificity.
Biopolymers - 1 Jan 2001
Ting A Y, Witte K, Shah K, Kraybill B, Shokat K M, Schultz P G
Abstract excerpt
The problem of identifying downstream targets of kinase phosphorylation remains a challenge despite technological advances in genomics and proteomics. A recent approach involves the generation of kinase mutants that can uniquely use "orthogonal" ATP analogs to phosphorylate substrates in vivo. Using structure-based design, mutants of several protein kinase superfamily members have been found; robust and general...
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