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Article

Ligand-induced movements of inner transmembrane helices of Glut1 revealed by chemical cross-linking of di-cysteine mutants.

PloS one - 1 Jan 2012

Mueckler Mike, Makepeace Carol

Abstract excerpt

The relative orientation and proximity of the pseudo-symmetrical inner transmembrane helical pairs 5/8 and 2/11 of Glut1 were analyzed by chemical cross-linking of di-cysteine mutants. Thirteen functional di-cysteine mutants were created from a C-less Glut1 reporter construct containing cysteine substitutions in helices 5 and 8 or helices 2 and 11. The mutants were expressed in Xenopus oocytes and the sensitivity...

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Identifiers

PMID: 22363641
DOI: 10.1371/journal.pone.0031412
PMCID: PMC3282689

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