Article
Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support.
FEBS letters - 14 Dec 2001
Katayama K, Dobashi Y, Kitagawa M, Kamekura S, Kawai M, Kadoya Y, Kameya T
Abstract excerpt
The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly...
Topics
- Animals
- Apoptosis
- Bromodeoxyuridine
- Cell Size
- Cyclin D1
- Cyclin-Dependent Kinase 4
- Cyclin-Dependent Kinase Inhibitor p16
- Cyclin-Dependent Kinases
- Flow Cytometry
- Fluorescein-5-isothiocyanate
- G1 Phase
- Gene Expression
- Genes, Dominant
- Mutation
- PC12 Cells
- Proto-Oncogene Proteins
- Rats
- Time Factors