Article
Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi.
Biochemistry - 8 Oct 1996
MacLeod A M, Tull D, Rupitz K, Warren R A, Withers S G
Abstract excerpt
The exoglucanase/xylanase Cex from Cellulomonas fimi is a retaining glycosidase which functions via a two-step mechanism involving the formation and hydrolysis of a covalent glycosyl-enzyme intermediate. The roles of three conserved active site carboxylic acids in this enzyme have been probed by...
Topics
- Binding Sites
- Catalysis
- Cellobiose
- Endo-1,4-beta Xylanases
- Glucosides
- Gram-Positive Asporogenous Rods
- Hydrogen-Ion Concentration
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Proteins
- Xylosidases