Substitution of Gly-96 to Ala in the 5-enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae results in a greatly reduced affinity for the herbicide glyphosate.

Abstract excerpt

The aroA gene of Klebsiella pneumoniae encoding the shikimate pathway enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, which is the target of the herbicide glyphosate, was cloned and sequenced from both the wild-type and the glyphosate-resistant mutant K. pneumoniae K1, which possesses a glyphosate-insensitive EPSP synthase. Both genes were expressed in Escherichia coli and were capable of complementing...

Topics

• 3-Phosphoshikimate 1-Carboxyvinyltransferase
• Alanine
• Alkyl and Aryl Transferases
• Amino Acid Sequence
• Base Sequence
• DNA, Bacterial
• Drug Resistance, Microbial
• Glycine
• Herbicides
• Klebsiella pneumoniae