Article
How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli.
Planta - 1 Nov 2002
Eschenburg Susanne, Healy Martha L, Priestman Melanie A, Lushington Gerald H, Schönbrunn Ernst
Abstract excerpt
The enzyme 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19) is essential for the biosynthesis of aromatic compounds in plants and microbes and is the unique target of the herbicide glyphosate. One of the first glyphosate-insensitive enzymes reported was a Gly96Ala mutant of EPSP synthase from Klebsiella pneumoniae. We have introduced this single-site mutation into the highly homologous EPSP...
Topics
- 3-Phosphoshikimate 1-Carboxyvinyltransferase
- Alanine
- Alkyl and Aryl Transferases
- Amino Acid Substitution
- Binding Sites
- Drug Resistance, Bacterial
- Escherichia coli
- Glycine
- Kinetics
- Klebsiella pneumoniae
- Models, Molecular
- Mutation
- Phosphoenolpyruvate