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Article

Thr90 is a key residue of the bacteriorhodopsin proton pumping mechanism.

FEBS letters - 23 Nov 2001

Perálvarez A, Barnadas R, Sabés M, Querol E, Padrós E

Abstract excerpt

Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of Asp85 (about 1.3 pH units higher than wild type). Hydroxylamine accessibility is increased in both...

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Identifiers

PMID: 11728460
DOI: 10.1016/s0014-5793(01)03080-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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