Article
Effect of genetic modification of tyrosine-185 on the proton pump and the blue-to-purple transition in bacteriorhodopsin.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Jang D J, el-Sayed M A, Stern L J, Mogi T, Khorana H G
Abstract excerpt
The retinylidene chromophore mutant (Y185F) of bacteriorhodopsin, in which Tyr-185 is substituted by phenylalanine, is examined and compared with wild-type bacteriorhodopsin expressed in Escherichia coli; both were reinstituted similarly in vesicles. The Y185F mutant shows (at least) two distinct...
Topics
- Bacteriorhodopsins
- Biological Transport, Active
- Hydrogen-Ion Concentration
- Light
- Mutation
- Pigments, Biological
- Retinaldehyde
- Spectrum Analysis
- Structure-Activity Relationship
- Tyrosine