Article
Analysis of the cell-dependent replication potentials of human immunodeficiency virus type 1 vif mutants.
Microbes and infection - 1 Nov 2001
Fujita M, Sakurai A, Doi N, Miyaura M, Yoshida A, Sakai K, Adachi A
Abstract excerpt
Eleven in-frame vif gene mutants of HIV type 1 produced in non-permissive cells were examined for their replication potentials in various CD4-positive and -negative cell lines. Virus replication for each mutant was monitored by using several single- and multiple-cycle infectivity assays. Except for a mutant with wild-type phenotype, most mutants were severely defective for replication in all the cell lines as...
Topics
- Amino Acid Sequence
- Blotting, Western
- Cloning, Molecular
- DNA, Viral
- Gene Products, vif
- HIV-1
- HeLa Cells
- Humans
- Jurkat Cells
- Kinetics
- Molecular Sequence Data
- Mutation
- Virus Replication
- vif Gene Products, Human Immunodeficiency Virus