Article
Roles of the aromatic side chains in the binding of substrates, inhibitors, and cyclomalto-oligosaccharides to the glucoamylase from Aspergillus niger probed by perturbation difference spectroscopy, chemical modification, and mutagenesis.
Carbohydrate research - 6 Apr 1992
Svensson B, Sierks M R
Abstract excerpt
The roles of the aromatic side chains of the glucoamylase from Aspergillus niger in the binding of ligands, as determined by difference spectroscopy using four types of inhibitors (a) valienamine-derived, (b) 1-deoxynojirimycins, (c) D-glucono-1,5-lactone, and (d) maltitol, two types of disacchar...
Topics
- Aspergillus niger
- Binding Sites
- Carbohydrate Sequence
- Glucan 1,4-alpha-Glucosidase
- Ligands
- Molecular Sequence Data
- Mutation
- Spectrophotometry, Ultraviolet
- Structure-Activity Relationship
- Substrate Specificity