Article
A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction.
The American journal of pathology - 1 Nov 2001
Szuhai K, Sandhaus E, Kolkman-Uljee S M, Lemaître M, Truffert J C, Dirks R W, Tanke H J, Fleuren G J, Schuuring E, Raap A K
Abstract excerpt
Human papillomaviruses (HPVs) play an important role in the pathogenesis of cervical cancer. For identification of the large number of different HPV types found in (pre)malignant lesions, a robust methodology is needed that combines general HPV detection with HPV genotyping. We have developed for formaldehyde-fixed samples a strategy that, in a homogeneous, real-time fluorescence polymerase chain reaction...
Topics
- Animals
- Cell Line
- Cell Nucleus
- DNA, Viral
- Female
- Fluorescent Dyes
- Gene Dosage
- Genome
- Genotype
- HeLa Cells
- Humans