Article
Detection and genotyping of human papillomavirus by real-time PCR assay.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Mar 2013
Moreau Frédérique, Fetouchi Rachid, Micalessi Isabelle, Brejeon Valérie, Bacon Nathalie, Jannes Geert, Le Pendeven Catherine, Lekbaby Bouchra, Kremsdorf Dina, Lacau Saint Guily Jean, Soussan Patrick
Abstract excerpt
BACKGROUND: Diagnosis of human papillomavirus (HPV) disease remains a challenge due to several factors related to the cost, the workload of available commercial assays to detect and genotype HPV, and to the low prevalence of infected patients. OBJECTIVE: Our study aimed to develop a real-time PCR, based on SPF10 primers, in order to combine HPV-DNA detection and genotype identification avoiding the negative...
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