Article
A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] - 1 Mar 2020
Nikouyan Negin, Farhadi Ali, Gorzin Ali Akbar, Geramizadeh Bita, Okhovat Mohammad Ali, Seyyedi Noorossadat, Dehbidi Gholamreza Rafiei, Ranjbaran Reza, Behzad-Behbahani Abbas
Abstract excerpt
Early diagnosis and genotyping of high-risk human papillomavirus (HR-HPV) in cervical tissue specimens is significant for cervical cancer prevention. A sensitive microplate fluorometric hybridization assay (MFHA) was designed for the detection of HPV DNA 16 and 18 in cervical tissue. Following optimization and validation of the method, 60 formalin-fixed and paraffin-embedded cervical samples representing...
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