Article
DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry.
Nucleic acids research - 1 Nov 2001
Edwards J R, Itagaki Y, Ju J
Abstract excerpt
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) has been explored widely for DNA sequencing. The major requirement for this method is that the DNA sequencing fragments must be free from alkaline and alkaline earth salts as well as other contaminants for accurately measuring the masses of the DNA fragments. We report here the development of a novel MS DNA sequencing method that...
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