Article
Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells.
BioTechniques - 1 Oct 2001
Petrulis J R, Perdew G H
Abstract excerpt
A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to...
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