Article
Assessing regulated nuclear transport in Saccharomyces cerevisiae.
Methods in cell biology - 1 Jan 2014
Ptak Christopher, Wozniak Richard W
Abstract excerpt
Regulated protein transport between the cytoplasm and the nucleoplasm occurs through nuclear pore complexes and is critical to the function of numerous biological pathways. Saccharomyces cerevisiae has been used as a model system to probe the underlying mechanisms of nuclear transport and how they regulate various physiological processes. This has been facilitated, in part, by studies that couple the microscopic...
Topics
- Active Transport, Cell Nucleus
- Amino Acid Motifs
- Cell Cycle Checkpoints
- Gene Expression
- Green Fluorescent Proteins
- Guanine Nucleotide Exchange Factors
- Karyopherins
- Membrane Transport Proteins
- Microscopy, Fluorescence
- Monomeric GTP-Binding Proteins
- Mutation
- Nuclear Pore
- Nuclear Pore Complex Proteins