Reconstitution of nuclear protein transport with semi-intact yeast cells.

doi:10.1083/jcb.123.4.785

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Article

Reconstitution of nuclear protein transport with semi-intact yeast cells.

The Journal of cell biology - 1 Nov 1993

Schlenstedt G, Hurt E, Doye V, Silver P A

Abstract excerpt

We have developed an in vitro nuclear protein import reaction from semi-intact yeast cells. The reaction uses cells that have been permeabilized by freeze-thaw after spheroplast formation. Electron microscopic analysis and antibody-binding experiments show that the nuclear envelope remains intact...

Topics

Antigens, Polyomavirus Transforming
Biological Transport, Active
Cell Nucleus
Membrane Glycoproteins
Microscopy, Electron
Mutation
Nuclear Localization Signals
Nuclear Pore Complex Proteins
Nuclear Proteins
Proteins
Saccharomyces cerevisiae

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