Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.

doi:10.1083/jcb.111.3.807

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Article

Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.

The Journal of cell biology - 1 Sept 1990

Adam S A, Marr R S, Gerace L

Abstract excerpt

We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to study biochemical events in the transport of macromolecules across the nuclear envelope. While treatment of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear en...

Topics

Amino Acid Sequence
Animals
Antigens, Polyomavirus Transforming
Biological Transport
Cytoplasm
Digitonin
HeLa Cells
Humans
In Vitro Techniques
Kidney
Kinetics
Liver
Molecular Sequence Data
Mutation
Nuclear Proteins
Oocytes
Peptides
Permeability

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