Article
Genetic synthetic lethality screen at the single gene level in cultured human cells.
Nucleic acids research - 15 Oct 2001
Simons A H, Dafni N, Dotan I, Oron Y, Canaani D
Abstract excerpt
Recently, we demonstrated the feasibility of a chemical synthetic lethality screen in cultured human cells. We now demonstrate the principles for a genetic synthetic lethality screen. The technology employs both an immortalized human cell line deficient in the gene of interest, which is complemented by an episomal survival plasmid expressing the wild-type cDNA for the gene of interest, and the use of a novel...
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