Article
The effect of mutations in the Xis-binding sites on site-specific recombination in coliphage HK022.
Molecular genetics and genomics : MGG - 1 Dec 2001
Gottfried P, Kolot M, Yagil E
Abstract excerpt
Excisionase (Xis) is an accessory protein that is required for the excision of the related prophages lambda and HK022. Xis binds to two tandemly arranged binding sites (X1 and X2) on the P arm of the recombination sites attP and attR. Gel-retardation analyses and site-specific recombination assays were conducted on derivatives bearing site-directed mutations in the X1 and X2 sites of phage HK022. The results...
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