Article
Random antisense cDNA mutagenesis as an efficient functional genomic approach in higher plants.
Planta - 1 Mar 2002
Jun Ji Hyung, Kim Cheol Soo, Cho Dae Shik, Kwak June Myoung, Ha Chan Man, Park Yu Shin, Cho Baik Ho, Patton David A, Nam Hong Gil
Abstract excerpt
Most cellular processes in an organism depend on functions of expressed sequences. Thus, efficient large-scale functional assignment of expressed sequences is crucial for understanding cellular processes. Towards this goal in plants, we designed a "random antisense cDNA mutagenesis (RAM)" approach. In a pilot experiment, 1,000 transgenic plants of Arabidopsis thaliana (L.) Heynh. (ecotype Wassilevskija)...
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