Article
A T-DNA mutant screen that combines high-throughput phenotyping with the efficient identification of mutated genes by targeted genome sequencing.
BMC plant biology - 4 Dec 2019
Frank Ulrike, Kublik Susanne, Mayer Dörte, Engel Marion, Schloter Michael, Durner Jörg, Gaupels Frank
Abstract excerpt
BACKGROUND: Nitrogen dioxide (NO2) triggers hypersensitive response (HR)-like cell death in Arabidopsis thaliana. A high-throughput mutant screen was established to identify genes involved in this type of programmed cell death. RESULTS: Altogether 14,282 lines of SALK T-DNA insertion mutants were screened. Growing 1000 pooled mutant lines per tray and simultaneous NO2 fumigation of 4 trays in parallel facilitated...
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