Article
The FOX hunting system: an alternative gain-of-function gene hunting technique.
The Plant journal : for cell and molecular biology - 1 Dec 2006
Ichikawa Takanari, Nakazawa Miki, Kawashima Mika, Iizumi Haruko, Kuroda Hirofumi, Kondou Youichi, Tsuhara Yumi, Suzuki Kumiko, Ishikawa Akie, Seki Motoaki, Fujita Miki, Motohashi Reiko, Nagata Noriko, Takagi Tomoko, Shinozaki Kazuo, Matsui Minami
Abstract excerpt
We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis...
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