Article
A heat-shock-activated cDNA encoding GAGA factor rescues some lethal mutations in the Drosophila melanogaster Trithorax-like gene.
Genetical research - 1 Aug 2001
Granok H, Leibovitch B A, Elgin S C
Abstract excerpt
GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic...
Topics
- Alleles
- Animals
- Bacterial Proteins
- Blotting, Western
- Crosses, Genetic
- DNA, Complementary
- DNA-Binding Proteins
- Drosophila Proteins
- Drosophila melanogaster
- Female
- Homeodomain Proteins
- Hot Temperature
- Male
- Models, Genetic
- Mutation
- Plasmids
- Protein Isoforms
- Recombination, Genetic