Article
Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes.
The Journal of molecular diagnostics : JMD - 1 Feb 2006
Liew Michael, Nelson Lesa, Margraf Rebecca, Mitchell Sheri, Erali Maria, Mao Rong, Lyon Elaine, Wittwer Carl
Abstract excerpt
High-resolution melting techniques are a simple and cost-effective alternative to other closed-tube genotyping methods. Here, we genotyped human platelet antigens (HPAs) 1 to 6 and 15 by high-resolution melting methods that did not require labeled probes. Conventional melting analysis with hybridization probes (HybProbes) was also performed at each locus. HybProbe assays were performed individually, whereas...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.