Article
Cytoplasmic expression of ribozyme in zebrafish using a T7 autogene system.
Current issues in molecular biology - 1 Jan 2001
Walker K, Xie Y, Li Y, Zhu Q, Xu W, Wagner T E, Chen X
Abstract excerpt
A cytoplasmic ribozyme expression system, based on codelivery of a ribozyme vector, a T7 autogene vector, and T7 RNA polymerase (RNAP), has been developed and used to generate a specific phenotype in zebrafish by targeting a no tail (ntl) mRNA. The expression of the no tail ribozyme sequence is under the control of a tandem of two promoters: The T7 promoter and an adenoviral va 1 (pol III) promoter. The...
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