Article
Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique.
Molecular biology reports - 1 Jun 2008
Pei De-Sheng, Sun Yong-Hua, Long Yong, Zhu Zuo-Yan
Abstract excerpt
External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the...
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