Article
[Rapid detection of drug resistant HIV -1 to reverse transcriptase and protease inhibitors in HIV-infected patients receiving highly active antiretroviral therapies].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Dec 2000
Yu M, Kabat W, Wang J
Abstract excerpt
OBJECTIVE: To develop and validate a rapid and sensitive method that can be used to detect emergence of HIV-1 drug resistant viruses in HIV-infected patients receiving highly active antiretroviral therapies (HAART). METHODS: HIV-1 viral RNA was extracted from plasma and amplified by nested RT-PCR. Nucleotide sequences of the reverse transcriptase (RT) and protease (PR) genes were determined by automated DNA...
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