Article
In vitro selection of exonic splicing enhancer sequences: identification of novel CD44 enhancers.
Nucleic acids research - 1 Aug 2001
Woerfel G, Bindereif A
Abstract excerpt
We have developed an in vitro selection procedure that allows the identification and isolation of functional splicing enhancer sequences from any cDNA. It is based on the enhancement of general splicing activity of a pre-mRNA reporter derived from the Drosophila dsx gene. Short DNase I fragments are cloned into a cassette in the second exon of the reporter construct, replacing the natural dsx enhancer. After...
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