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Article

Identification of Mycobacterial Species by Comparative Sequence Analysis of the RNA Polymerase Gene ( <i>rpoB</i> )

1 Jun 1999

Abstract excerpt

For the differentiation and identification of mycobacterial species, the rpoB gene, encoding the beta subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 44 reference strains of mycobacteria and clinical isolates (107 strains) by PCR. The nucleotide sequences were directly determined (306 bp) and aligned by using the multiple alignment algorithm in the MegAlign package (DNASTAR)...

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PMID: 10325313
DOI: 10.1128/jcm.37.6.1714-1720.1999

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