Article
An enhanced mutant of red fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation.
The Journal of biological chemistry - 10 Aug 2001
Verkhusha V V, Otsuna H, Awasaki T, Oda H, Tsukita S, Ito K
Abstract excerpt
We developed a new variant of coral-derived red fluorescent protein, DsRed S197Y, which is brighter and essentially free from secondary fluorescence peak. This makes it an ideal reporter for double labeling with green fluorescent protein (GFP). Though purified protein shows only 20% stronger fluorescence emission, culture cells that express DsRed S197Y exhibit a 3-3.5 times higher level of fluorescence than the...
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