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Article

Isolation, expression, and characterization of fully functional nontoxic BiP/GRP78 mutants.

Protein expression and purification - 1 Jun 2001

King L S, Berg M, Chevalier M, Carey A, Elguindi E C, Blond S Y

Abstract excerpt

Mammalian BiP/GRP78 and Escherichia coli DnaK belong to the highly conserved hsp70 family and function as molecular chaperones in the endoplasmic reticulum or the cytosol, respectively. Induction of murine BiP/GRP78 expression in E. coli leads to growth arrest and cell death, independent of the bacterial strain and vector used. Analysis of various BiP constructs and mutants shows that the dominant-lethal...

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Identifiers

PMID: 11388813
DOI: 10.1006/prep.2001.1424

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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