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Efficient production of antibodies against a mammalian integral membrane protein by phage display

2 Aug 2011

Abstract excerpt

The application of phage display technology to mammalian proteins with multiple transmembrane regions has had limited success due to the difficulty in generating these proteins in sufficient amounts and purity. We report here a method that can be easily and generally applied to sorting of phage display libraries with multispan protein targets solubilized in detergent. A key feature of this approach is the...

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PMID: 21810920
DOI: 10.1093/protein/gzr039

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