Article
Site-directed mutagenesis evidence for a negatively charged trypsin inhibitory loop in sweet potato sporamin.
FEBS letters - 11 May 2001
Yao P L, Hwang M J, Chen Y M, Yeh K W
Abstract excerpt
Sporamin, a sweet potato tuberous storage protein, has trypsin inhibitory activity. Sequence comparison with other plant trypsin inhibitors (TIs) of the Kunitz family reveals that, instead of the conserved Arg or Lys found in other Kunitz TIs, sporamin contains a negatively charged residue (Asp70 or Glu72) at the P1 reactive site. Using site-directed mutagenesis, six mutants were generated containing...
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