Article
Cloning, expression, and complementation test of the RNA lariat debranching enzyme cDNA from mouse.
Molecules and cells - 30 Apr 2001
Kim H C, Kim G M, Yang J M, Ki J W
Abstract excerpt
The RNA lariat debranching enzyme of mouse (mDBR1) was cloned by screening a NIH/3T3 cDNA library. The sequence of full-length mDBR1 cDNA contained a single 515 amino acid open reading frame of 58 kDa protein. Comparison of the amino acid sequence of mDBR1 to other DBR proteins showed 40%, 44%, 43%, 42%, and 80% identity to Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans, Drosophila...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Blotting, Southern
- Cloning, Molecular
- DNA, Complementary
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- Genes, Fungal
- Genetic Complementation Test