Article
Isolation and characterization of the gene encoding yeast debranching enzyme.
Cell - 3 May 1991
Chapman K B, Boeke J D
Abstract excerpt
Using a genetic screen aimed at identifying cellular factors involved in Ty1 transposition, we have identified a mutation in a host gene that reduces Ty1 transposition frequency. The mutant, dbr1, is also defective in the process of intron turnover. In dbr1 cells, excised introns derived from a variety of pre-mRNAs are remarkably stable and accumulate to levels exceeding that of the corresponding mRNA. The stable...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Transposable Elements
- Introns
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Open Reading Frames
- RNA Nucleotidyltransferases
- RNA Precursors
- RNA, Fungal