Article
Four-color flow cytometric detection of retrovirally expressed red, yellow, green, and cyan fluorescent proteins.
BioTechniques - 1 May 2001
Hawley T S, Telford W G, Ramezani A, Hawley R G
Abstract excerpt
Flow cytometric procedures are described to detect a "humanized" version of a new red fluorescent protein (DsRed) from the coral Discosoma sp. in conjunction with various combinations of three Aequorea victoria green fluorescent protein (GFP) variants--EYFP, EGFP, and ECFP. In spite of overlapping emission spectra, the combination of DsRed with EYFP, EGFP, and ECFP generated fluorescence signals that could be...
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