Article
Simultaneous analysis of the cyan, green, and yellow fluorescent proteins.
Current protocols in cytometry - 1 May 2001
Beavis A J, Kalejta R F
Abstract excerpt
Flow cytometric analysis of fluorescent protein expressing cells is of particular interest to researchers in many areas. The detection of fluorescent proteins in cells allows one to monitor gene expression, determine intracellular protein localization, and identify transfected cells. Wild-type green fluorescent protein has limited utility as its spectral properties are not suitable for standard cytometers....
Topics
- Animals
- Bacterial Proteins
- Cell Separation
- Equipment Design
- Flow Cytometry
- Fluorescent Dyes
- Green Fluorescent Proteins
- Humans
- Luminescent Proteins
- Mutagenesis, Site-Directed
- Mutation