Four color immunofluorescence detection using two 488-nm lasers on a Becton Dickinson FACS Vantage flow cytometer.

Article

Four color immunofluorescence detection using two 488-nm lasers on a Becton Dickinson FACS Vantage flow cytometer.

Cytometry - 1 Oct 1997

DeMaria M, Johnson R P, Rosenzweig M

Abstract excerpt

Multiparameter flow cytometric analysis is sometimes limited by the availability of directly conjugated monoclonal antibodies or streptavidin-conjugated secondary reagents. While many manufacturers offer a wide variety of monoclonal antibodies or streptavidin reagents directly conjugated to 488-n...

Topics

Antigens, CD
Cell Separation
Flow Cytometry
Fluorescent Antibody Technique
Fluorescent Dyes
Granulocytes
Humans
In Vitro Techniques
Lasers
Lymphocytes
Monocytes
Phenotype

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