Article
Metaphase arrest with centromere separation in polo mutants of Drosophila.
The Journal of cell biology - 14 May 2001
Donaldson M M, Tavares A A, Ohkura H, Deak P, Glover D M
Abstract excerpt
The Drosophila gene polo encodes a conserved protein kinase known to be required to organize spindle poles and for cytokinesis. Here we report two strongly hypomorphic mutations of polo that arrest cells of the larval brain at a point in metaphase when the majority of sister kinetochores have separated by between 20-50% of the total spindle length in intact cells. In contrast, analysis of sister chromatid...
Topics
- Anaphase
- Animals
- Brain
- Cdc20 Proteins
- Cell Cycle Proteins
- Centromere
- Cyclin A
- Cyclin B
- Drosophila
- Drosophila Proteins
- Larva
- Metaphase
- Mutation
- Neurons
- Protein Kinases
- Protein Serine-Threonine Kinases
- Sister Chromatid Exchange
- Spindle Apparatus