High‐level accumulation of a recombinant antibody fragment in the periplasm of <i>Escherichia coli</i> requires a triple‐mutant (<i>degP prc spr</i>) host strain

Abstract excerpt

During production of a humanized antibody fragment secreted into the periplasm of Escherichia coli, proteolytic degradation of the light chain was observed. In order to determine which protease(s) were responsible for this degradation, we compared expression of the F(ab')(2) antibody fragment in several E. coli strains carrying mutations in genes encoding periplasmic proteases. Analysis of strains cultured in...

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